Quantitative gene expression analysis

Quantitative gene expression analysis

Manager


Jean-Christophe Helbling

Objective


To provide high-performance tools for measuring and comparing gene expression in various biological contexts, with the aim of elucidating the molecular mechanisms underlying physiological and pathological processes.

Presentation


The laboratory uses advanced technologies to accurately assess gene expression levels. These approaches are adapted to different biological models and can meet specific needs in basic or applied research.

Our expertise

In addition to classical analyses, the laboratory also excels in specialized techniques for the study of specific cell populations and their RNAs:

  • Total RNA extraction: from tissues or cellular samples.
  • Targeted RNA purification by pS6-TRAP and viral-TRAP: these approaches enable the isolation of cell-specific RNAs actively translating proteins, offering a precise view of regulatory mechanisms at the translational level.
  • RNA assay by absorbance on microvolumes: for precise quantification of nucleic acids.
  • RNA quality control: assessment of sample integrity prior to analysis.
  • cDNA synthesis: preparation for mRNA quantification.
  • Relative quantification of mRNA: use of robust techniques such as quantitative real-time PCR (qPCR) or high-throughput sequencing (in collaboration with PGTB, INRAE Cestas-Pierroton).
  • Optimization of experimental protocols: support in the design and validation of experiments to guarantee reliable and reproducible results.
  • Comparative and statistical analysis: bioinformatics expertise to interpret results within a rigorous framework.

Available equipment

The laboratory is equipped with state-of-the-art instruments to guarantee accurate and reliable analyses:

  • Qiagen TissueLyser II: for efficient tissue grinding prior to extraction.
  • NanoDrop One: for rapid, precise nucleic acid assay on micro-volumes.
  • Bioanalyzer 2100 Agilent: for quality control of nucleic acids and verification of their integrity.
  • Eppendorf epMotion 5070 pipetting robot: for automated preparation of qPCR plates, guaranteeing optimum precision.
  • Roche LightCycler 480: for flexible qPCR in 96- or 384-well plates.
  • Versatile thermal cyclers: for conventional PCR and cDNA synthesis in 96-well plates or tubes.

Our strengths

  • Laboratory equipped with the latest technologies in molecular analysis: including advanced technical skills such as pS6-TRAP and viral-TRAP.
  • Staff with expertise in interpreting complex data.
  • Customized approach based on the objectives of each project.