Protein characterization and quantification

Manager: Jean-Christophe Helbling

Objective

To provide high-performance tools for the extraction, characterization and quantification of proteins in order to study their roles in various biological contexts and elucidate the molecular mechanisms involved in complex pathophysiological processes.

Presentation

The laboratory offers a full range of techniques for protein analysis, adapted to various types of biological samples. These approaches enable precise and reproducible characterization of proteins, from extraction to specific detection.

Our expertise

We master advanced methods to meet your protein analysis needs:

  • Protein extraction and quantification: from tissues (especially brain structures), isolated cells, subcellular compartments or specific microdomains such as rafts.
  • Western blot: for specific identification and quantification of proteins of interest.
  • Immunoprecipitation: to isolate and study specific protein complexes.
  • Fluorescence analysis of proteins with the Odyssey system: a technology based on fluorescence detection for precise and sensitive quantification, developed in collaboration with the Magendie biochemistry platform (INSERM).

Available equipment

Our laboratory relies on high-performance equipment, complemented by collaborations with specialized platforms:

  • Qiagen TissueLyser II: for mechanical sample preparation prior to protein extraction.
  • NanoDrop One: for rapid protein quantification.
  • GelDoc Bio-Rad: for imaging and analysis of Western gels and membranes.
  • Odyssey LI-COR: for high-sensitivity fluorescence detection, enabling multi-parameter analysis of tagged proteins.

Our strengths

  • Sample flexibility: brain structures, single cells, subcellular fractions and rafts.
  • Advanced technologies: including fluorescence detection with the LI-COR Odyssey, in partnership with the Magendie biochemistry platform (INSERM).
  • Scientific expertise: support in designing experiments and interpreting protein data.

Possible applications

  • Study of protein expression profiles in normal and pathological conditions.
  • Analysis of protein-protein interactions.
  • Identification and characterization of specific biomarkers.