Microscopy – Image analysis

Managers: Jean-Christophe Delpech and Alexandra Sere

Applications

• Immunohistochemistry
• In situ hybridization
• Epifluorescence imaging
• Transmitted light imaging
• Automated wide-field acquisition
• Phagocytic activity study
• Dendritic spine counting

Equipment

Microscopes

• Zeiss Axio Observer7 with apotome3, colibri 7 LED system, AI sample finder, equipped with an Axiocam 305 color camera and an Axiocam 712 mono fluorescent camera (Zen software with Intellesis+ImAnalysis modules)
• Leica Med IHC-MICR-0,5 equipped with a Nikon DXM1200 digital camera (Nikon ACT-X software) and an Olympus BX51TF-5
• Nikon Eclipse E400, equipped with Hamamatsu orca-R² camera and piezzo (Nikon NIS Element AR software)
• Olympus BX51TF microscope, equipped for Lucia with a Nikon
• DXM 1200 digital camera for Lucia and PixelLink digital camera and X,Y position sensors for Mercator
• BIC platform: macroscope, confocal, multiphoton, FRET, FRAP, STED, PALM, metamorph software, etc.

Image analysis software

Zen with Intellesis analysis module (analysis approach with machine learning)
Image J/Fiji (free image analysis software)
Imaris (3D image analysis – images acquired in fluorescence), Lucia (morphology, grayscale),
Mercator (cartography, stereology)
Metamorph