{"id":156,"date":"2025-01-17T09:46:38","date_gmt":"2025-01-17T08:46:38","guid":{"rendered":"https:\/\/nutrineuro-prod.onlinecreation.pro\/resources-and-competences\/electrophysiologie-sur-tranche-de-cerveau\/"},"modified":"2025-01-17T09:48:52","modified_gmt":"2025-01-17T08:48:52","slug":"electrophysiologie-sur-tranche-de-cerveau","status":"publish","type":"page","link":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/resources-and-competences\/electrophysiologie-sur-tranche-de-cerveau\/","title":{"rendered":"Electrophysiologie sur tranche de cerveau"},"content":{"rendered":"\n<p>Responsables : Cl\u00e9mentine Bosch-Bouju, Xavier Fioramonti, David Jarriault<\/p>\n\n<h3 class=\"wp-block-heading\">Applications<\/h3>\n\n<ul class=\"wp-block-list\">\n<li>Mesure de la transmission et de la plasticit\u00e9 synaptique<\/li>\n\n\n\n<li>Mesure des param\u00e8tres \u00e9lectrophysiologiques et de l\u2019excitabilit\u00e9 des neurones sur tranche de cerveaux de rongeurs<\/li>\n\n\n\n<li>Mesure de la sensibilit\u00e9 de neurones aux nutriments et hormones<\/li>\n\n\n\n<li>Etudes sp\u00e9cifiques de neurones particuliers gr\u00e2ce \u00e0 l\u2019utilisation de \u00ab souris fluorescentes \u00bb (NPY-GFP, POMC-GFP, D1R-GFP, D2R-GFP, Pet1-mCherry, CxCR1-GFP, etc.)<\/li>\n\n\n\n<li>Structures c\u00e9r\u00e9brales \u00e9tudi\u00e9es : hippocampe, cortex, noyau accumbens, striatum, raph\u00e9 dorsal, noyaux hypothalamiques<\/li>\n<\/ul>\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"155\" height=\"125\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image9.jpeg\" alt=\"\" class=\"wp-image-143\"\/><figcaption class=\"wp-element-caption\">Image en infrarouge (haut) et en fluorescence (bas) de neurones s\u00e9rotonine (5-HT) dans le Raph\u00e9 dorsal de souris Pet1-cre\/mCherry<\/figcaption><\/figure>\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"88\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image10.png\" alt=\"\" class=\"wp-image-145\"\/><figcaption class=\"wp-element-caption\">Enregistrement \u00e9lectrique en courant-clamp d\u2019un neurone NPY-GFP en r\u00e9ponse \u00e0 des variations de la concentration en glucose.<\/figcaption><\/figure>\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"196\" height=\"69\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image11.jpeg\" alt=\"\" class=\"wp-image-147\"\/><figcaption class=\"wp-element-caption\">Enregistrement \u00e9lectrique en voltage-clamp des courants inhibiteurs GABA spontan\u00e9s sur les neurones 5-HT du Raph\u00e9 dorsal en r\u00e9ponse \u00e0 l\u2019insuline<\/figcaption><\/figure>\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"80\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image12.jpeg\" alt=\"\" class=\"wp-image-149\"\/><figcaption class=\"wp-element-caption\">Stimulation optog\u00e9n\u00e9tique de neurones POMC du noyau arqu\u00e9 de souris POMC-cre inject\u00e9e avec un AAV-flex-hCHR2-mCherry<\/figcaption><\/figure>\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"103\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image13.jpeg\" alt=\"\" class=\"wp-image-151\"\/><figcaption class=\"wp-element-caption\">Enregistrement \u00e9lectrophysiologique d\u2019un neurone moyen \u00e9pineux du striatum chez la souris. A, Profil \u00e9lectrophysiologique du neurone suite \u00e0 l\u2019injection de sauts de courants (incr\u00e9ment 10 pA). B, R\u00e9ponse synaptique du neurone suite \u00e0 la stimulation des aff\u00e9rences glutamatergiques (2 stimulations \u00e0 50 ms d\u2019intervalle)  <\/figcaption><\/figure>\n\n<h3 class=\"wp-block-heading\">Appareillages<\/h3>\n\n<ul class=\"wp-block-list\">\n<li>3 postes d\u2019\u00e9lectrophysiologie patch-clamp sur tranche de cerveaux<\/li>\n\n\n\n<li>Microscopes droits avec lumi\u00e8re blanche, infrarouge et fluorescence (LEDs combinables 370 nm, 460nm, 580nm)<\/li>\n\n\n\n<li>Stimulation \u00e9lectrique<\/li>\n\n\n\n<li>Stimulation optog\u00e9n\u00e9tique<\/li>\n<\/ul>\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"444\" height=\"135\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image14.jpeg\" alt=\"\" class=\"wp-image-153\" srcset=\"https:\/\/nutrineuro.bordeaux.inrae.fr\/wp-content\/uploads\/sites\/25\/2025\/01\/image14.jpeg 444w, https:\/\/nutrineuro.bordeaux.inrae.fr\/wp-content\/uploads\/sites\/25\/2025\/01\/image14-300x91.jpeg 300w\" sizes=\"auto, (max-width: 444px) 100vw, 444px\" \/><figcaption class=\"wp-element-caption\">Photos de la salle d\u2019\u00e9lectrophysiologie NutriEphy<\/figcaption><\/figure>\n\n<p><\/p>\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Responsables : Cl\u00e9mentine Bosch-Bouju, Xavier Fioramonti, David Jarriault Applications Appareillages<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":88,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-156","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/pages\/156","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/comments?post=156"}],"version-history":[{"count":1,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/pages\/156\/revisions"}],"predecessor-version":[{"id":157,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/pages\/156\/revisions\/157"}],"up":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/pages\/88"}],"wp:attachment":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/fr\/wp-json\/wp\/v2\/media?parent=156"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}