{"id":173,"date":"2025-01-17T10:24:08","date_gmt":"2025-01-17T09:24:08","guid":{"rendered":"https:\/\/nutrineuro-prod.onlinecreation.pro\/?page_id=173"},"modified":"2025-01-17T10:27:37","modified_gmt":"2025-01-17T09:27:37","slug":"protein-characterization-and-quantification","status":"publish","type":"page","link":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/resources-and-competences\/protein-characterization-and-quantification\/","title":{"rendered":"Protein characterization and quantification"},"content":{"rendered":"\n<p>Manager: Jean-Christophe Helbling<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Objective<\/h3>\n\n\n\n<p>To provide high-performance tools for the extraction, characterization and quantification of proteins in order to study their roles in various biological contexts and elucidate the molecular mechanisms involved in complex pathophysiological processes.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Presentation<\/h3>\n\n\n\n<p>The laboratory offers a full range of techniques for protein analysis, adapted to various types of biological samples. These approaches enable precise and reproducible characterization of proteins, from extraction to specific detection.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Our expertise<\/h3>\n\n\n\n<p>We master advanced methods to meet your protein analysis needs:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Protein extraction and quantification:<\/strong> from tissues (especially brain structures), isolated cells, subcellular compartments or specific microdomains such as rafts.<\/li>\n\n\n\n<li><strong>Western blot:<\/strong> for specific identification and quantification of proteins of interest.<\/li>\n\n\n\n<li><strong>Immunoprecipitation:<\/strong> to isolate and study specific protein complexes.<\/li>\n\n\n\n<li><strong>Fluorescence analysis of proteins with the Odyssey system:<\/strong> a technology based on fluorescence detection for precise and sensitive quantification, developed in collaboration with the Magendie biochemistry platform (INSERM).<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Available equipment<\/h3>\n\n\n\n<p>Our laboratory relies on high-performance equipment, complemented by collaborations with specialized platforms:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Qiagen TissueLyser II:<\/strong> for mechanical sample preparation prior to protein extraction.<\/li>\n\n\n\n<li><strong>NanoDrop One:<\/strong> for rapid protein quantification.<\/li>\n\n\n\n<li><strong>GelDoc Bio-Rad:<\/strong> for imaging and analysis of Western gels and membranes.<\/li>\n\n\n\n<li><strong>Odyssey LI-COR:<\/strong> for high-sensitivity fluorescence detection, enabling multi-parameter analysis of tagged proteins.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Our strengths<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Sample flexibility:<\/strong> brain structures, single cells, subcellular fractions and rafts.<\/li>\n\n\n\n<li><strong>Advanced technologies:<\/strong> including fluorescence detection with the LI-COR Odyssey, in partnership with the Magendie biochemistry platform (INSERM).<\/li>\n\n\n\n<li><strong>Scientific expertise:<\/strong> support in designing experiments and interpreting protein data.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Possible applications<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Study of protein expression profiles in normal and pathological conditions.<\/li>\n\n\n\n<li>Analysis of protein-protein interactions.<\/li>\n\n\n\n<li>Identification and characterization of specific biomarkers.<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Manager: Jean-Christophe Helbling Objective To provide high-performance tools for the extraction, characterization and quantification of proteins in order to study [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":18,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-173","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/173","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/comments?post=173"}],"version-history":[{"count":1,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/173\/revisions"}],"predecessor-version":[{"id":174,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/173\/revisions\/174"}],"up":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/media?parent=173"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}