{"id":168,"date":"2025-01-17T10:11:55","date_gmt":"2025-01-17T09:11:55","guid":{"rendered":"https:\/\/nutrineuro-prod.onlinecreation.pro\/?page_id=168"},"modified":"2025-01-17T10:11:56","modified_gmt":"2025-01-17T09:11:56","slug":"quantitative-gene-expression-analysis","status":"publish","type":"page","link":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/resources-and-competences\/quantitative-gene-expression-analysis\/","title":{"rendered":"Quantitative gene expression analysis"},"content":{"rendered":"\n<p>Quantitative gene expression analysis<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Manager<\/h3>\n\n\n\n<p>\u2028Jean-Christophe Helbling<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Objective<\/h3>\n\n\n\n<p>\u2028To provide high-performance tools for measuring and comparing gene expression in various biological contexts, with the aim of elucidating the molecular mechanisms underlying physiological and pathological processes.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Presentation\u2028<\/h3>\n\n\n\n<p>The laboratory uses advanced technologies to accurately assess gene expression levels. These approaches are adapted to different biological models and can meet specific needs in basic or applied research.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Our expertise<\/h3>\n\n\n\n<p>In addition to classical analyses, the laboratory also excels in specialized techniques for the study of specific cell populations and their RNAs:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Total RNA extraction:<\/strong> from tissues or cellular samples.<\/li>\n\n\n\n<li><strong>Targeted RNA purification by pS6-TRAP and viral-TRAP:<\/strong> these approaches enable the isolation of cell-specific RNAs actively translating proteins, offering a precise view of regulatory mechanisms at the translational level.<\/li>\n\n\n\n<li><strong>RNA assay by absorbance on microvolumes:<\/strong> for precise quantification of nucleic acids.<\/li>\n\n\n\n<li><strong>RNA quality control:<\/strong> assessment of sample integrity prior to analysis.<\/li>\n\n\n\n<li><strong>cDNA synthesis:<\/strong> preparation for mRNA quantification.<\/li>\n\n\n\n<li><strong>Relative quantification of mRNA:<\/strong> use of robust techniques such as quantitative real-time PCR (qPCR) or high-throughput sequencing (in collaboration with PGTB, INRAE Cestas-Pierroton).<\/li>\n\n\n\n<li><strong>Optimization of experimental protocols:<\/strong> support in the design and validation of experiments to guarantee reliable and reproducible results.<\/li>\n\n\n\n<li><strong>Comparative and statistical analysis:<\/strong> bioinformatics expertise to interpret results within a rigorous framework.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Available equipment<\/h3>\n\n\n\n<p>The laboratory is equipped with state-of-the-art instruments to guarantee accurate and reliable analyses:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Qiagen TissueLyser II:<\/strong> for efficient tissue grinding prior to extraction.<\/li>\n\n\n\n<li><strong>NanoDrop One:<\/strong> for rapid, precise nucleic acid assay on micro-volumes.<\/li>\n\n\n\n<li><strong>Bioanalyzer 2100 Agilent:<\/strong> for quality control of nucleic acids and verification of their integrity.<\/li>\n\n\n\n<li><strong>Eppendorf epMotion 5070 pipetting robot:<\/strong> for automated preparation of qPCR plates, guaranteeing optimum precision.<\/li>\n\n\n\n<li><strong>Roche LightCycler 480:<\/strong> for flexible qPCR in 96- or 384-well plates.<\/li>\n\n\n\n<li><strong>Versatile thermal cyclers:<\/strong> for conventional PCR and cDNA synthesis in 96-well plates or tubes.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Our strengths<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Laboratory equipped with the latest technologies in molecular analysis:<\/strong> including advanced technical skills such as pS6-TRAP and viral-TRAP.<\/li>\n\n\n\n<li><strong>Staff with expertise<\/strong> in interpreting complex data.<\/li>\n\n\n\n<li><strong>Customized approach<\/strong> based on the objectives of each project.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Quantitative gene expression analysis Manager \u2028Jean-Christophe Helbling Objective \u2028To provide high-performance tools for measuring and comparing gene expression in various [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":18,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-168","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/168","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/comments?post=168"}],"version-history":[{"count":1,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/168\/revisions"}],"predecessor-version":[{"id":169,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/168\/revisions\/169"}],"up":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/media?parent=168"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}