{"id":142,"date":"2025-01-17T09:46:38","date_gmt":"2025-01-17T08:46:38","guid":{"rendered":"https:\/\/nutrineuro-prod.onlinecreation.pro\/?page_id=142"},"modified":"2025-01-17T09:46:39","modified_gmt":"2025-01-17T08:46:39","slug":"electrophysiology-on-brain-slices","status":"publish","type":"page","link":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/resources-and-competences\/electrophysiology-on-brain-slices\/","title":{"rendered":"Electrophysiology on brain slices"},"content":{"rendered":"\n<p>Managers: Cl\u00e9mentine Bosch-Bouju, Xavier Fioramonti, David Jarriault<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Applications<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Measurement of synaptic transmission and plasticity<\/li>\n\n\n\n<li>Measurement of electrophysiological parameters and neuronal excitability on rodent brain slices<\/li>\n\n\n\n<li>Measurement of neuron sensitivity to nutrients and hormones<\/li>\n\n\n\n<li>Specific studies of particular neurons using \u201cfluorescent mice\u201d (NPY-GFP, POMC-GFP, D1R-GFP, D2R-GFP, Pet1-mCherry, CxCR1-GFP, etc.).<\/li>\n\n\n\n<li>Brain structures studied: hippocampus, cortex, nucleus accumbens, striatum, dorsal raphe, hypothalamic nuclei<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"155\" height=\"125\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image9.jpeg\" alt=\"\" class=\"wp-image-143\"\/><figcaption class=\"wp-element-caption\">Infrared (top) and fluorescence (bottom) imaging of serotonin (5-HT) neurons in the dorsal raphe of Pet1-cre\/mCherry mice<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"88\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image10.png\" alt=\"\" class=\"wp-image-145\"\/><figcaption class=\"wp-element-caption\">Current-clamp electrical recording of a NPY-GFP neuron in response to variations in glucose concentration.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"196\" height=\"69\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image11.jpeg\" alt=\"\" class=\"wp-image-147\"\/><figcaption class=\"wp-element-caption\">Voltage-clamp electrical recording of spontaneous GABA inhibitory currents on dorsal raphe 5-HT neurons in response to insulin<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"80\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image12.jpeg\" alt=\"\" class=\"wp-image-149\"\/><figcaption class=\"wp-element-caption\">Optogenetic stimulation of POMC neurons in the arcuate nucleus of POMC-cre mice injected with AAV-flex-hCHR2-mCherry<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"103\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image13.jpeg\" alt=\"\" class=\"wp-image-151\"\/><figcaption class=\"wp-element-caption\">Electrophysiological recording of a striatal middle spiny neuron in mice. A, Electrophysiological profile of the neuron following injection of current jumps (10 pA increment). B, Synaptic response of the neuron following stimulation of glutamatergic afferents (2 stimulations at 50 ms intervals).<\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Equipment<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>3 brain slice patch-clamp electrophysiology stations<\/li>\n\n\n\n<li>Upright microscopes with white light, infrared and fluorescence (combinable LEDs 370 nm, 460nm, 580nm)<\/li>\n\n\n\n<li>Electrical stimulation<\/li>\n\n\n\n<li>Optogenetic stimulation<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"444\" height=\"135\" src=\"https:\/\/nutrineuro-prod.onlinecreation.pro\/wp-content\/uploads\/sites\/25\/2025\/01\/image14.jpeg\" alt=\"\" class=\"wp-image-153\" srcset=\"https:\/\/nutrineuro.bordeaux.inrae.fr\/wp-content\/uploads\/sites\/25\/2025\/01\/image14.jpeg 444w, https:\/\/nutrineuro.bordeaux.inrae.fr\/wp-content\/uploads\/sites\/25\/2025\/01\/image14-300x91.jpeg 300w\" sizes=\"auto, (max-width: 444px) 100vw, 444px\" \/><figcaption class=\"wp-element-caption\">Photos of the NutriEphy electrophysiology room<\/figcaption><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Managers: Cl\u00e9mentine Bosch-Bouju, Xavier Fioramonti, David Jarriault Applications Equipment<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":18,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-142","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/142","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/comments?post=142"}],"version-history":[{"count":1,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/142\/revisions"}],"predecessor-version":[{"id":155,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/142\/revisions\/155"}],"up":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/media?parent=142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}