{"id":138,"date":"2025-01-17T09:33:03","date_gmt":"2025-01-17T08:33:03","guid":{"rendered":"https:\/\/nutrineuro-prod.onlinecreation.pro\/?page_id=138"},"modified":"2025-01-17T09:33:15","modified_gmt":"2025-01-17T08:33:15","slug":"genotyping-of-transgenic-lines","status":"publish","type":"page","link":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/resources-and-competences\/genotyping-of-transgenic-lines\/","title":{"rendered":"Genotyping of transgenic lines"},"content":{"rendered":"\n<p>Manager: Jean-Christophe Helbling<\/p>\n\n\n\n<p>Operational team: C\u00e9line Ducroix-Cr\u00e9py et Eva Bruchet<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Goal<\/h3>\n\n\n\n<p>\u2028To ensure the reproduction, management and selection of transgenic lines in the Nutrineuro laboratory, using rapid and reliable genotyping techniques adapted to optimal colony management.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Our skills<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Rapid DNA extraction:<\/strong> from mouse tail biopsies (5 mm).<\/li>\n\n\n\n<li><strong>Simplex and multiplex PCR techniques:<\/strong> for targeted and simultaneous amplification of several genetic markers.<\/li>\n\n\n\n<li><strong>Agarose gel electrophoresis:<\/strong> to visualize amplification results.<\/li>\n\n\n\n<li><strong>Hot Start Taq Pol polymerization enzyme:<\/strong> an innovation produced and purified directly by our team for increased reliability and efficiency.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Applications<\/h3>\n\n\n\n<p>The genotyping division offers specific PCRs for line discrimination and pre-birth sexing:<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Line organization<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Management and monitoring of transgenic lines using the ANISE interface, facilitating precise traceability of crosses and genotypes.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Equipment available<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Applied Biosystems 2720 thermal cycler: for simplex and multiplex PCR.<\/li>\n\n\n\n<li>Agilent thermocycler 8800 overcycler technology<\/li>\n\n\n\n<li>Applied simpliAmp Applied biosystems thermocycler<\/li>\n\n\n\n<li>Lifeeco Bioer thermal cycler<\/li>\n\n\n\n<li>E-BOX imaging system, Vilber: for visualization and analysis of agarose gels.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Highlights<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast, cost-effective approach to genotyping large colonies.<\/li>\n\n\n\n<li>In-house innovation with Hot Start Taq Pol enzyme.<\/li>\n\n\n\n<li>Optimized line management with dedicated tools, ANISE web interface.<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Manager: Jean-Christophe Helbling Operational team: C\u00e9line Ducroix-Cr\u00e9py et Eva Bruchet Goal \u2028To ensure the reproduction, management and selection of transgenic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":18,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-138","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/138","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/comments?post=138"}],"version-history":[{"count":1,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/138\/revisions"}],"predecessor-version":[{"id":139,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/138\/revisions\/139"}],"up":[{"embeddable":true,"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/pages\/18"}],"wp:attachment":[{"href":"https:\/\/nutrineuro.bordeaux.inrae.fr\/en\/wp-json\/wp\/v2\/media?parent=138"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}